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2018創新技術博覽會

技術查詢及媒合洽談 




技術名稱:

罕見細胞篩選晶片暨自動化影像分析系統 Rare cell screening chip and automatic image analysis system


單位/學校:

國立清華大學 NATIONAL TSING HUA UNIVERSITY


技術說明:

本技術包含有細胞自組裝陣列晶片(Self-Assemblely Cells Array, SACA)、自動化影像分析系統、細胞針抓取等三項技術組合成「微量細胞篩選晶片暨自動化影像分析系統」。 其核心技術SACA chip是一個單純利用重力、蒸散力、液體側向拉力形成大面積單層排列細胞之微流體晶片,每一個晶片具有8個well,可在5分鐘內攤平約2cc全血進行觀察。由於其低物理傷害特性,經過混入並重新篩選之tumor cell line存活率高達90%,標準螢光辨識(2 positive, 1 negative)輔助下可在1:107的低濃度下達到85%的檢出率,再搭配自動化影像系統擷取細胞在晶片中的數量、形貌、相對位置等等資訊(約40分鐘)。最後可使用細胞針抓取配件將選定之微量細胞取出進行後續的分析應用,例如培養、基因分析、藥物毒理測試等等。 「Rare cell screening chip and automatic image analysis system」 consist of a Self-Assemblely Cells Array (SACA) chip, an automated image analysis system, and a cell needle grabbing. The major technology, SACA chip, is a microfluidic chip that uses gravity, evapotranspiration, and liquid lateral force to form large area single-layer cells. Each chip has 8 wells and can spread 2ml of whole blood in 5 minutes. Due to its low physical damage characteristics, the spiked tumor cell line has survival rate up to 90%, and the recover rate reach 85% under concentration 1:107 with standard fluorescence identification (2 positive, 1 negative). After that, the automated imaging system observe the numbers, morphology and relative position of the cells in the chip within 40 minutes. Finally, the selected micro-cells can be removed by cell needle grasping accessories for subsequent analysis, such as culture, genetic analysis or drug toxicology etc..


發明人:

曾繁根 特聘教授


連絡資訊:

賴淑真 03-5715131

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